Was able to get CLC to recognize Annotated oyster genome when converting mRNA only GFF name to gene.
-3 in AltSPlice folder





Also took full gff and converted "mRNA" to "gene" and left "CDS" as is  (-4)




Both miss exon info
will change CDS -> exon (-5)


FINALLY!

GFF has to annotated as gene and mRNA
oyster.v9.gene_mRNA.gff







Running Gil on v9_90






All data @
http://eagle.fish.washington.edu/cnidarian/AltSpliceCLC_030613.zip








Transcript Level Expression

Mgo_1 (paired) RNA-Seq_transcript.txt
Gil_1 (paired) RNA-Seq_transcript.txt